Human Genetics

, Volume 34, Issue 2, pp 199–206

An improved technique for selective silver staining of nucleolar organizer regions in human chromosomes

Authors

  • S. E. Bloom
    • Department of Poultry ScienceCornell University
    • Department of BiologyM. D. Anderson Hospital and Tumor Institute
  • C. Goodpasture
    • Department of Poultry ScienceCornell University
    • Department of BiologyM. D. Anderson Hospital and Tumor Institute
Original Investigations

DOI: 10.1007/BF00278889

Cite this article as:
Bloom, S.E. & Goodpasture, C. Hum. Genet. (1976) 34: 199. doi:10.1007/BF00278889

Summary

A reliable technique for staining human chromosomal nucleolar organizers (NOR's) with silver solutions is described. The NOR's can be selectively stained dark brown by silver solutions leaving the chromosome arms unstained and available for counterstaining with orcein or Giemsa dyes. Unequivocal identification of chromosome pairs bearing NOR's can be achieved using fluorescent banding techniques followed by silver staining. The silver staining procedure for NOR's was simplified and standardized through control of the chemical and physical conditions during silver impregnation and developing.

Copyright information

© Springer-Verlag 1976