Molecular and General Genetics MGG

, Volume 105, Issue 4, pp 306–313

Biochemical and genetic classification of riboflavine deficient mutants of Saccharomyces cerevisiae

  • O. Oltmanns
  • A. Bacher
  • F. Lingens
  • F. K. Zimmermann
Article

DOI: 10.1007/BF00277585

Cite this article as:
Oltmanns, O., Bacher, A., Lingens, F. et al. Molec. Gen. Genet. (1969) 105: 306. doi:10.1007/BF00277585

Summary

Twenty six riboflavine deficient mutants of Saccharomyces cerevisiae were isolated. They can be divided by biochemical methods into four classes, accumulating (i) no specific product (S), (ii) 2,5-diamino-6-hydroxy-4-ribitylaminopyrimidine (AP), (iii) 5-amino-2,6-dihydroxy-4-ribitylaminopyrimidine (HP), (iv) 6,7-dimethyl-8-ribityllumazine (LU).

In genetic experiments six complementation groups were found. Complementation group I is congruent with class S, group II with AP, III and IV with HP and V and VI with LU. In the crosses tested so far, no linkage between complementation groups could be detected by tetrad analysis. Intragenic complementation was found within complementation group V (mutants accumulating LU).

Copyright information

© Springer-Verlag 1969

Authors and Affiliations

  • O. Oltmanns
    • 1
  • A. Bacher
    • 1
  • F. Lingens
    • 1
  • F. K. Zimmermann
    • 2
  1. 1.Institut für Mikrobiologie und Molekularbiologie der Universität HohenheimStuttgart-Hohenheim
  2. 2.Forstbotanisches Institut der Universität Freiburg i. Br.Freiburg i. Br.Germany