Molecular and General Genetics MGG

, Volume 240, Issue 2, pp 181–187

Chromosome mapping in Alealigenes eutrophus CH34

  • Abdelkrim Sadouk
  • Max Mergeay
Article

DOI: 10.1007/BF00277055

Cite this article as:
Sadouk, A. & Mergeay, M. Molec. Gen. Genet. (1993) 240: 181. doi:10.1007/BF00277055

Abstract

Mutants and mobilizing plasmids were developed as genetic tools in Alcaligenes eutrophus CH34. In order to map the chromosome, spontaneous and ethyl methane sulphonate (EMS)-induced mutants (mostly auxotrophs) were isolated. Another source of mutants was provided by the phenomenon of temperature-induced mortality and mutagenesis that is observed at 37° C and is characteristic of many metallotolerant strains of A. eutrophus. Plasmid pULB113 (RP4::miniMu) was used to map the available mutations. Twenty-five loci were ordered in a circular map. pMOL50, a rearranged derivative of plasmid pMOL28, which was obtained in a survivor at 37° C and displayed chromosome mobilizing activity (Cma+), was also used to mobilize chromosomal markers: resulting linkages were stronger than with pULB113, allowing confirmation of the circularity of the A. eutrophus CH34 chromosome with a small number of crosses.

Key words

Chromosome mapping Conjugative plasmids Alcaligenes eutrophus 

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Abdelkrim Sadouk
    • 1
  • Max Mergeay
    • 1
  1. 1.Laboratoire de Génétique et BiotechnologieCentre d'Etudes de l'Energie Nucléaire and Vlaamse Instelling voor Technologisch Onderzoek, SCK/CEN-VITOBoeretangBelgium

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