Molecular and General Genetics MGG

, Volume 240, Issue 1, pp 73–80

A nucleotide substitution in one of the β-tubulin genes of Trichoderma viride confers resistance to the antimitotic drug methyl benzimidazole-2-yl-carbamate

  • G. H. Goldman
  • W. Temmerman
  • D. Jacobs
  • R. Contreras
  • M. Van Montagu
  • A. Herrera-Estrella
Original Articles

DOI: 10.1007/BF00276886

Cite this article as:
Goldman, G.H., Temmerman, W., Jacobs, D. et al. Molec. Gen. Genet. (1993) 240: 73. doi:10.1007/BF00276886

Abstract

We characterized a Trichoderma viride strain that is resistant to the antimitotic drug methyl benzimidazole-2-yl-carbamate (MBC). This species has two β-tubulin genes (tub1 and tub2) and by reverse genetics we showed that a mutation in the tub2 gene confers MBC resistance in this strain. Comparison of the tub2 sequence of the mutant strain with that of the wild type revealed that a single amino acid substitution of tyrosine for histidine at position 6 is responsible for the MBC tolerance. Furthermore, we showed that this gene can be used as a homologous dominant selectable marker in T. viride transformation. Both tubulin genes were completely sequenced. They differ by 48 residues and the degree of identity between their deduced amino acid sequences is 86.3%.

Key words

BenzimidazolesFilamentous fungiResistanceTrichodermaTubulins

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • G. H. Goldman
    • 1
  • W. Temmerman
    • 1
  • D. Jacobs
    • 1
  • R. Contreras
    • 2
  • M. Van Montagu
    • 1
  • A. Herrera-Estrella
    • 1
  1. 1.Laboratorium voor GeneticaUniversiteit GentGentBelgium
  2. 2.Laboratorium voor Moleculaire BiologieUniversiteit GentGentBelgium
  3. 3.Department of PharmacologyUMDNJ-Robert Wood Johnson Medical SchoolPiscatawayUSA
  4. 4.Centro de Investigacion y Estudios AvanzadosIrapuatoMexico