Archives of Microbiology

, Volume 158, Issue 6, pp 412–417

Maleylacetate reductase of Pseudomonas sp. strain B13: dechlorination of chloromaleylacetates, metabolites in the degradation of chloroaromatic compounds

  • Stefan R. Kaschabek
  • Walter Reineke
Original Papers

DOI: 10.1007/BF00276301

Cite this article as:
Kaschabek, S.R. & Reineke, W. Arch. Microbiol. (1992) 158: 412. doi:10.1007/BF00276301

Abstract

The maleylacetate reductase of 3-chlorobenzoate-grown cells of Pseudomonas sp. strain B13 has been purified 50-fold. The enzyme converted 2-chloromaleylacetate to 3-oxoadipate with temporary occurrence of maleylacetate; 1 mol of chloride was eliminated during the conversion of 1 mol of 2-chloro- and 2,3-dichloromaleylacetate; 2 mol of NADH were consumed per mol of 2-chloro- and 2,3-dichloromaleylacetate while only 1 mol was necessary to catalyze the conversion of maleylacetate or 2-methylmaleylacetate. The maleylacetate reductase failed to use fumarylacetate as a substrate. The role of the enzyme in the chloroaromatics degradation is discussed.

Key words

2-Chloromaleylacetate2,3-dichloromaleylacetatePseudomonas sp. strain B13Maleylacetate reductase

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Stefan R. Kaschabek
    • 1
  • Walter Reineke
    • 1
  1. 1.Chemische Mikrobiologie der Bergischen Universität — Gesamthochschule WuppertalWuppertal 1Federal Republic of Germany