Molecular and General Genetics MGG

, Volume 227, Issue 3, pp 417–423

Analysis of yeast prp20 mutations and functional complementation by the human homologue RCC1, a protein involved in the control of chromosome condensation

  • Martin Fleischmann
  • Michael W. Clark
  • Wayne Forrester
  • Marvin Wickens
  • Takeharu Nishimoto
  • Markus Aebi
Article

DOI: 10.1007/BF00273932

Cite this article as:
Fleischmann, M., Clark, M.W., Forrester, W. et al. Molec. Gen. Genet. (1991) 227: 417. doi:10.1007/BF00273932

Summary

Mutations in the PRP20 gene of yeast show a pleiotropic phenotype, in which both mRNA metabolism and nuclear structure are affected. srm1 mutants, defective in the same gene, influence the signal transduction pathway for the pheromone response. The yeast PRP20/SRM1 protein is highly homologous to the RCC1 protein of man, hamster and frog. In mammalian cells, this protein is a negative regulator for initiation of chromosome condensation. We report the analysis of two, independently isolated, recessive temperature-sensitive prp20 mutants. They have identical G to A transitions, leading to the alteration of a highly conserved glycine residue to glutamic acid. By immunofluorescence microscopy the PRP20 protein was localized in the nucleus. Expression of the RCC1 protein can complement the temperature-sensitive phenotype of prp20 mutants, demonstrating the functional similarity of the yeast and mammalian proteins.

Key words

Saccharomyces cerevisiae mRNA metabolism Nucleus 

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Martin Fleischmann
    • 1
  • Michael W. Clark
    • 2
  • Wayne Forrester
    • 3
  • Marvin Wickens
    • 3
  • Takeharu Nishimoto
    • 4
  • Markus Aebi
    • 1
  1. 1.Institut für Molekularbiologie IUniversität ZürichZürichSwitzerland
  2. 2.Department of BiologyMcGill UniversityMontrealCanada
  3. 3.Department of Biochemistry, College of Agriculture and Life ScienceUniversity of WisconsinMadisonUSA
  4. 4.Department of Molecular Biology, Graduate School of Medical ScienceKyushu UniversityFukuokaJapan

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