Human Genetics

, Volume 80, Issue 4, pp 397–398

Assignment of human coagulation factor XII (fXII) to chromosome 5 by cDNA hybridization to DNA from somatic cell hybrids

  • F. Citarella
  • M. Tripodi
  • A. Fantoni
  • F. Bernardi
  • G. Romeo
  • M. Rocchi
Short Communications

DOI: 10.1007/BF00273661

Cite this article as:
Citarella, F., Tripodi, M., Fantoni, A. et al. Hum Genet (1988) 80: 397. doi:10.1007/BF00273661

Summary

Human coagulation factor XII (fXII), a serine protease synthesized in liver and active in plasma, is involved in a wide variety of functions, including blood coagulation, fibrinolysis, bradykinin and complement activation. A complementary DNA (597 bp) encoding amino acid-16 to amino acid 183 of fXII protein was used to determine the chromosomal location of the fXII gene. DNAs from hamster-human somatic cell hybrids were digested with restriction enzymes and hybridized with the fXII cDNA. By the Southern method it was shown that restriction fragments able to hybridize to fXII cDNA are present only in DNA extracted from clones retaining human chromosome 5.

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • F. Citarella
    • 1
  • M. Tripodi
    • 1
  • A. Fantoni
    • 1
  • F. Bernardi
    • 2
  • G. Romeo
    • 3
  • M. Rocchi
    • 3
  1. 1.Sezione di Biologia Cellulare, Dipartimento di Biopatologia UmanaUniversità di Roma “La Sapienza”RomeItaly
  2. 2.Centro Studi Biochimici Morbo di CooleyUniversità di FerraraFerraraItaly
  3. 3.Laboratorio di Genetica MolecolareIstituto G. GasliniGenovaItaly