Histochemistry

, Volume 93, Issue 6, pp 655–660

Immunohistochemistry of endogenous l-DOPA in the rat posterior hypothalamus

  • F. Tison
  • N. Mons
  • M. Geffard
  • P. Henry
Article

DOI: 10.1007/BF00272209

Cite this article as:
Tison, F., Mons, N., Geffard, M. et al. Histochemistry (1990) 93: 655. doi:10.1007/BF00272209

Summary

The aim of this work was to study l-DOPA-containing neuronal structures of the rat posterior and dorsal hypothalamus by means of immunohistochemistry using antiserum against glutaraldehyde conjugated l-DOPA. Aspects and distribution of l-DOPA immunoreaction among cells of the supramammillary nucleus and the A11, A13c and A13 cell groups are described and compared to dopamine immunoreactivity, mainly through a double colored labelling procedure employing a color modification of the DAB reaction by metallic ions. Differences between l-DOPA and dopamine stainings within cell groups as the presence of cells with predominant or exclusive l-DOPA coloration are tentatively explained under the light of previous findings using immunohistochemistry of catecholamines synthesizing enzymes and catecholamines histofluorescence.

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • F. Tison
    • 1
    • 2
  • N. Mons
    • 2
  • M. Geffard
    • 2
  • P. Henry
    • 1
  1. 1.Département de NeurologieHôpital PellegrinBordeaux CédexFrance
  2. 2.Laboratoire d'Immunologie, IBCN-CNRSUniversité de Bordeaux IIBordeaux CédexFrance