Molecular and General Genetics MGG

, Volume 229, Issue 2, pp 301–306

Construction of a fusion gene comprising the Taka-amylase A promoter and the Escherichia coli β-glucuronidase gene and analysis of its expression in Aspergillus oryzae

  • Setsuzo Tada
  • Katsuya Gomi
  • Katsuhiko Kitamoto
  • Kojiro Takahashi
  • Gakuzo Tamura
  • Shodo Hara
Article

DOI: 10.1007/BF00272170

Cite this article as:
Tada, S., Gomi, K., Kitamoto, K. et al. Molec. Gen. Genet. (1991) 229: 301. doi:10.1007/BF00272170

Summary

Northern blot analysis of glucose-grown and starch-grown mycelia of Aspergillus oryzae R11340 was conducted using the cloned Taka-amylase A (TAA) gene as a probe. The amount of mRNA homologous to the TAA gene was increased when this fungus was grown with starch as a sole carbon source. In order to analyze the induction mechanism, we inserted the Escherichia coli uidA gene encoding β-glucuronidase (GUS) downstream of the TAA promoter and introduced the resultant fusion gene into the A. oryzae genome. Production of a functional GUS protein was induced by starch, but not by glucose. When the effects of various sugars on expression of the fusion gene were examined, the results suggested that the expression of the fusion gene was under control of the TAA gene promoter.

Key words

Aspergillus oryzaeTaka-amylase AGene expressionβ-Glucuronidase (uidA)Fusion gene

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Setsuzo Tada
    • 1
  • Katsuya Gomi
    • 2
  • Katsuhiko Kitamoto
    • 2
  • Kojiro Takahashi
    • 2
  • Gakuzo Tamura
    • 1
  • Shodo Hara
    • 2
  1. 1.Research Institute of Brewing Resources Co. Ltd.Kita-ku, TokyoJapan
  2. 2.National Research Institute of BrewingKita-ku, TokyoJapan
  3. 3.Kirin Brewery Co., Ltd.Technical centerTsurumi-ku, Yokohama-shiJapan