Molecular and General Genetics MGG

, Volume 163, Issue 3, pp 241–255

Physical mapping of genes on yeast mitochondrial DNA: Localization of antibiotic resistance loci, and rRNA and tRNA genes

  • Richard Morimoto
  • Sylvie Merten
  • Alfred Lewin
  • Nancy C. Martin
  • Murray Rabinowitz
Article

DOI: 10.1007/BF00271954

Cite this article as:
Morimoto, R., Merten, S., Lewin, A. et al. Molec. Gen. Genet. (1978) 163: 241. doi:10.1007/BF00271954
  • 40 Downloads

Summary

We have physically mapped the loci conferring resistance to antibiotics that inhibit mitochondrial protein synthesis (erythromycin, chloramphenicol and paromomycin) or respiration (oligomycin I and II), as well as the 21s and 14s rRNA and tRNA genes on the restriction map of the mitochondrial genome of the yeast Saccharomyces cerevisiae. The mitochondrial genes were localized by hybridization of labeled RNA probes to restriction fragments of grande (strain MH41-7B) mitochondrial DNA (mtDNA)1 generated by endonucleases EcoRI, HpaI, BamHI, HindIII, SalI, PstI and HhaI. We have derived the HhaI restriction fragment map of MH41-7B mit DNA, to be added to our previously reported maps for the six other endonucleases.

The antibiotic resistance loci (antR) were mapped by hybridization of 3H-cRNA transcribed from single marker petite mtDNA's of low kinetic complexity to grande restriction fragments. We have chosen the single Sal I site as the origin of the circular physical map and have positioned the antibiotic loci as follows: C (99.5-1.Ou)-P(27-36.Ou)-OII (58.3-62u)-OI (80-84u)-E (94.4-98.4u). The 21s rRNA is localized at 94.4-99.2u, and the 14s rRNA is positioned between 36.2-39.8u. The two rRNA species are separated by 36% of the genome. Total mitochondrial tRNA labeled with 125I hybridized primarily to two regions of the genome, at 99.5-11.5u and 34-44u. A third region of hybridization was occasionally detected at 70-76u, which probably corresponds to seryl and glutamyl tRNA genes, previously located to this region by petite deletion mapping.

Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • Richard Morimoto
    • 1
    • 2
    • 3
    • 4
  • Sylvie Merten
    • 1
    • 2
    • 3
    • 4
  • Alfred Lewin
    • 1
    • 2
    • 3
    • 4
  • Nancy C. Martin
    • 1
    • 2
    • 3
    • 4
  • Murray Rabinowitz
    • 1
    • 2
    • 3
    • 4
  1. 1.Department of BiologyThe University of Chicago Pritzker School of MedicineChicagoUSA
  2. 2.Department of MedicineThe University of Chicago Pritzker School of MedicineChicagoUSA
  3. 3.Department of BiochemistryThe University of Chicago Pritzker School of MedicineChicagoUSA
  4. 4.Franklin McLean Memorial Research InstituteChicagoUSA
  5. 5.Department of BiochemistryUniversity of Minnesota School of MedicineMinneapolis

Personalised recommendations