Applied Microbiology and Biotechnology

, Volume 20, Issue 5, pp 326–330

Cellulolytic complex of Aspergillus niger under conditions for citric acid production. Isolation and characterization of two β-(1→4)-glucan hydrolases

  • Smilja Vidmar
  • Vito Turk
  • Igor Kregar
Biotechnology

DOI: 10.1007/BF00270594

Cite this article as:
Vidmar, S., Turk, V. & Kregar, I. Appl Microbiol Biotechnol (1984) 20: 326. doi:10.1007/BF00270594

Summary

Two β-(1→4)-endoglucanases have been purified from industrial waste broth of Aspergillus niger grown under conditions which produce citric acid. Molecular weights for endoglucanase A were 43,000 and 25,000 for endoglucanase B. Both enzymes exhibited very similar properties: a rather broad pH optimum between pH 2 and 7 for CM-cellulose hydrolysis and an inability to degrade crystalline cellulose. The endoglucanases have a higher thermal stability at acid pH (up to 60°C) than at alkaline pH. They are inhibited by iodine, HgCl2 and N-bromosuccinimide.

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Smilja Vidmar
    • 1
  • Vito Turk
    • 1
  • Igor Kregar
    • 1
  1. 1.Department of Biochemistry, J. Stefan InstituteUniversity E. KardeljLjubljanaYugoslavia