Summary
A Pseudomonas sp. which was isolated from phenol-containing soil was immobilized in alginate and polyacrylamide-hydrazide (PAAH) and cultivated in a special airlift fermenter.
The immobilized Pseudomonas sp. was able to degrade phenol at initial concentrations up to 2 g/l in less than 2 days, although the free cells did not grow at this concentration.
The immobilization materials act as a protective cover against phenol, PAAH being more effective than alginate. The degradation activity as well as the outgrowth of bacteria can be manipulated by the concentration of the immobilization material, the temperature and the nitrogen content in the medium.
The cells grew predominantly in microcolonies in the outer area of the beads when nitrogen was available as 1.0g NH4NO3/l and 0.5g (NH4)2SO4/l.
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Prof. Dr. A. Fiechter dedicated to his 60th birthday
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Bettmann, H., Rehm, H.J. Degradation of phenol by polymer entrapped microorganisms. Appl Microbiol Biotechnol 20, 285–290 (1984). https://doi.org/10.1007/BF00270587
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DOI: https://doi.org/10.1007/BF00270587