Molecular and General Genetics MGG

, Volume 225, Issue 2, pp 257–265

Mutations in a Saccharomyces cerevisme host showing increased holding stability of the heterologous plasmid pSRI

  • Kenji Irie
  • Hiroyuki Araki
  • Yasuji Oshima
Article

DOI: 10.1007/BF00269857

Cite this article as:
Irie, K., Araki, H. & Oshima, Y. Molec. Gen. Genet. (1991) 225: 257. doi:10.1007/BF00269857

Summary

We have isolated Saccharomyces cerevisiae mutants, smp, showing stable maintenance of plasmid pSRI, a Zygosaccharomyces rouxii plasmid. The smp mutants were recessive and were classified into at least three different complementation groups. The three mutants also showed increased stability of YRp plasmids and the mutations are additive for plasmid stability. One mutation, smp1, confers a respiration-deficient (rho0) phenotype and several Rho mutants independently isolated by ethidium bromide treatment of the same yeast strain also showed increased stabilities of pSR1 and YRp plasmids. The wild-type S. cerevisiae cells showed a strongly biased distribution of pSR1 molecules as well as YRp plasmids to the mother cells at mitosis, while the smpf mutant did not show this bias. Another mutation, smp3, at a locus linked to ade2 on chromosome XV, confers temperature-sensitive growth. The SMP3 gene encodes a 59.9 kDa hydrophobic protein and disruption of the gene is lethal.

Key words

Plasmid stability pSRI plasmid rho mutation smp mutation Stable host 

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Kenji Irie
    • 1
  • Hiroyuki Araki
    • 1
  • Yasuji Oshima
    • 1
  1. 1.Department of Fermentation Technology, Faculty of EngineeringOsaka UniversitySuita-shi, OsakaJapan

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