Histochemistry

, Volume 102, Issue 6, pp 477–481

Improved in situ β-galactosidase staining for histological analysis of transgenic mice

Authors

  • A. Aguzzi
    • Institute of Neuropathology
  • F. Theuring
    • Schering Research Laboratories
Original Paper

DOI: 10.1007/BF00269579

Cite this article as:
Aguzzi, A. & Theuring, F. Histochemistry (1994) 102: 477. doi:10.1007/BF00269579

Abstract

The present study describes a novel method for the histochemical demonstration of β-galactosidase activity on tissue sections. We have replaced 5-bromo-4-chloro-3-indolyl-β-D-galactoside (X-Gal) with 5-bromoindolyl-β-o-galactopyranoside (Bluo-Gal) as a chromogenic substrate for the bacterial β-galactosidase (lacZ). After β-galactosidic cleavage, Bluo-Gal precipitates in form of fine birefringent crystals, whereas X-gal gives rise to an amorphous precipitate. Upon microscopic examination under polarized light, the crystals emit a strong signal consisting of yellow reflected light. This property of Bluo-Gal results in greatly enhanced sensitivity of the staining method for β-galactosidase and allows for optimal morphological resolution. To exemplify the applications of this technique, the expression is demonstrated in transgenic mice of β-galactosidase driven by a fragment of the human tissue-type plasminogen activator promoter.

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Copyright information

© Springer Verlag 1994