Molecular and General Genetics MGG

, Volume 143, Issue 1, pp 85–91

Location on the chromosome of Escherichia coli of genes governing purine metabolism

Adenosine deaminase (add), guanosine kinase (gsk) and hypoxanthine phosphoribosyltransferase (hpt)
  • Bjarne Jochimsen
  • Per Nygaard
  • Trygvi Vestergaard
Article

DOI: 10.1007/BF00269424

Cite this article as:
Jochimsen, B., Nygaard, P. & Vestergaard, T. Molec. Gen. Genet. (1975) 143: 85. doi:10.1007/BF00269424

Summary

Genes coding for enzymes functioning in purine salvage pathways have been located on the chromosome of Escherichia coli. The gene add encoding adenosine deaminase was located by transduction at 31 min, the gene order was established to be man-uidA-add-aroD. A deletion covering man-uidA-add was obtained. The gene gsk encoding guanosine kinase was cotransducible with purE and shown to be located at 13 min. The gene hpt encoding hypoxanthine phosphoribosyltransferase was contransducible with tonA indicating a location at 3 min. The location of the gene gpt encoding guanine (xanthine) phosphoribosyltransferase in the proA-proB region was confirmed.

Abbreviations

sAMP

succinyl-AMP

IMP

inosine monophosphate

XMP

xanthosine monophosphate

Ado

adenosine

Guo

guanosine

Ino

inosine

Ade

adenine

Hyp

hypoxanthine

Gua

guanine

Xan

xanthine

dAdo

deoxyadenosine

AI-CAR

5′ phosphoribosyl-5 amino-4 imidazole carboxamide

Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • Bjarne Jochimsen
    • 1
  • Per Nygaard
    • 1
  • Trygvi Vestergaard
    • 1
  1. 1.University Institute of Biological Chemistry BCopenhagenDenmark