Histochemistry

, Volume 99, Issue 3, pp 201–211

Cathepsin E in follicle associated epithelium of intestine and tonsils: localization to M cells and possible role in antigen processing

  • Giovanna Finzi
  • Matteo Cornaggia
  • Carlo Capella
  • Roberto Fiocca
  • Federica Bosi
  • Enrico Solcia
  • I. Michael Samloff
Article

DOI: 10.1007/BF00269138

Cite this article as:
Finzi, G., Cornaggia, M., Capella, C. et al. Histochemistry (1993) 99: 201. doi:10.1007/BF00269138

Abstract

A specific rabbit anti-human serum was used selectively to localize the aspartic proteinase cathepsin E to follicle associated epithelium (FAE) of human and rat intestine, including jejunum, ileum, appendix, colon and rectum, as well as of human palatine, pharyngeal and lingual tonsils. Coexpression of class II histocompatibility antigen HLA-DR antigen has been observed in some of the cathepsin E-positive epithelial cells. In addition, cathepsin E has been detected in a few mononuclear cells of intestinal lymphoid structures and tonsils resembling interdigitating reticulum cells of lymph nodes. Another aspartic proteinase, cathepsin D, has been found to be poorly represented in FAE and intensely expressed by macrophages. Electron immunocytochemistry localized cathepsin E to endosomal vesicles and endoplasmic reticulum of M cells in rat and human ileum as well as of M-like cells in human palatine tonsil. The results suggest a possible role of endosomal cathepsin E in the processing of macromolecules and microorganisms transported by M cells and related epithelial cells to mucosal associated lymphoid tissue (MALT).

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Giovanna Finzi
    • 1
  • Matteo Cornaggia
    • 1
  • Carlo Capella
    • 1
  • Roberto Fiocca
    • 2
  • Federica Bosi
    • 2
  • Enrico Solcia
    • 2
  • I. Michael Samloff
    • 3
    • 4
  1. 1.Department of Human Pathology and Genetics, II Medical FacultyUniversity of Pavia at Varese and Varese Multizonal HospitalVareseItaly
  2. 2.Department of Human Pathology and Genetics, I Medical FacultyUniversity of Pavia and IRCCS Policlinico S. MatteoPaviaItaly
  3. 3.Veterans Administration Medical CenterSepulvedaUSA
  4. 4.UCLA School of MedicineLos AngelesUSA
  5. 5.Dipartimento Patologia UmanaSezione Anatomia PatologicaPaviaItaly