Plant Cell Reports

, Volume 8, Issue 8, pp 479–482

Comparison of secondary product accumulation in photoautotrophic, photomixotrophic and heterotrophic Nicotiana tabacum cell suspension cultures

Authors

  • D. Ikemeyer
    • Lehrstuhl Biochemie der PflanzenWestfälische Wilhelms-Universität
  • W. Barz
    • Lehrstuhl Biochemie der PflanzenWestfälische Wilhelms-Universität
Article

DOI: 10.1007/BF00269053

Cite this article as:
Ikemeyer, D. & Barz, W. Plant Cell Reports (1989) 8: 479. doi:10.1007/BF00269053

Summary

Photoautotrophic, photomixotrophic and heterotrophic Nicotiana tabacum cell suspension cultures were compared for the constitutive accumulation of secondary metabolites and the elicitor-induced formation of the phytoalexin capsidiol. Nicotine and chlorogenic acid were found in high amounts in the heterotrophic cultures and in moderate concentrations in photomixotrophic but not in photoautotrophic cells. Nicotinic acid-N-glucoside occured in all culture types; in photoautotrophic and photomixotrophic cells the formation of N-methylnicotinic acid (trigonelline) was also observed. Treatment with a fungal elicitor led to substantial accumulation of capsidiol in heterotrophic and photomixotrophic cells and in only low levels in photoautotrophic cultures. Elicitor-treated photomixotrophic cells showed a pronounced increase in cell wall-bound phenolics. The levels of nicotine, nicotinic acid-N-glucoside and trigonelline were not affected by elicitation.

Key words

Nicotiana tabacumcell suspension culturenicotinechlorogenic acidnicotinic acid conjugateselicitationphotoautotrophy

Abbreviations

hcc

heterotrophic cell culture

mcc

photomixotrophic cell culture

pcc

photoautotrophic cell culture

fr.wt.

freshweight

nic-N-glc

nicotinic acid-N-glucoside

PMG

Phytophthora megasperma f. sp. glycínea

HPLC

high performance liquid chromatography

GC

gas chromatography

TLC

thin layer chromatography

2,4D

2,4-dichlorophenoxyacetic acid

Kin

kinetin

BAP

6-benzylaminopurine

NAA

α-naphthylacetic acid

Download to read the full article text

Copyright information

© Springer-Verlag 1989