Molecular and General Genetics MGG

, Volume 129, Issue 2, pp 167–176

Ribosomal protein neighborhoods

III. Cooperativity of assembly

Authors

  • L. C. Lutter
    • The Wallenberg LaboratoryUniversity of Uppsala
  • U. Bode
    • The Wallenberg LaboratoryUniversity of Uppsala
  • C. G. Kurland
    • The Wallenberg LaboratoryUniversity of Uppsala
  • G. Stöffler
    • Max-Planck-Institut für Molekulare Genetik
Article

DOI: 10.1007/BF00268629

Cite this article as:
Lutter, L.C., Bode, U., Kurland, C.G. et al. Molec. Gen. Genet. (1974) 129: 167. doi:10.1007/BF00268629

Summary

Diimidoesters have been used to cross-link neighboring proteins in the 30S ribosomal subunit of Escherichia coli. Two pairs of cross-linked proteins have been identified by several independent techniques; these are S7-S9 and S13-S19. Since it has been shown earlier that these four proteins cooperate during ribosome assembly in vitro (Green and Kurland, 1973), their identification as near neighbors in the ribosome is quite suggestive. Thus, the data indicate that a cluster of proteins which interact with each other during assembly would be, in general, found in close proximity in the functional ribosome. A more detailed discussion of the arrangement of the proteins in the 30S subunit is presented.

Download to read the full article text

Copyright information

© Springer-Verlag 1974