Plant Cell Reports

, Volume 6, Issue 3, pp 231–234

Somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L.)

  • Norma L. Trolinder
  • J. R. Goodin
Article

DOI: 10.1007/BF00268487

Cite this article as:
Trolinder, N.L. & Goodin, J.R. Plant Cell Reports (1987) 6: 231. doi:10.1007/BF00268487

Abstract

Tissue culture methods for improvement of cotton has lagged seriously compared to other major crops. A method for regeneration of cotton which includes a morphogenetically competent cell suspension was needed to facilitate selection of stress-resistant variants and gene manipulation. Preliminary screening of eight strains of Gossypium hirsutum L. for embryogenic potential resulted in the production of somatic embryos in all strains. Coker 312 was selected for use in the development of a model regeneration system for G. hirsutum. Calli were initiated from hypocotyl tissues of 3-day-old-seedlings. Globular embryos were present after six weeks in culture. Calli were subcultured to liquid suspension in growth regulator-free medium. After three to four weeks, suspensions were sieved to collect globular and heart stage embryos. Collected embryos developed further when plated onto semi-solid medium. To induce germination and plantlet growth, mature embryos were placed on sterile vermiculite saturated with medium. Upon development of roots and two true leaves, plantlets were potted in peat and sand, and hardened. Mature plants and progeny have been obtained with this procedure. A high percentage of infertile plants was observed among the regenerants.

Key Words

Gossypium hirsutum somatic embryogenesis 

Abbreviations

NAA

1 naphthaleneacetic acid

IAA

indole-3-acetic acid

2,4-D

2,4-dichlorophenoxyacetic acid

GA3

gibberellic acid

MS

Murashige and Skoog

BA

6 benzylamino purine

2i P

N6-(2-isopentenyladenine

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Norma L. Trolinder
    • 1
  • J. R. Goodin
    • 1
  1. 1.Department of Biological SciencesTexas Tech UniversityLubbockUSA
  2. 2.USDA-ARSLubbockUSA