Theoretical and Applied Genetics

, Volume 77, Issue 6, pp 786–792

Restriction fragment length polymorphism diversity in soybean

  • P. Keim
  • R. C. Shoemaker
  • R. G. Palmer
Originals

DOI: 10.1007/BF00268327

Cite this article as:
Keim, P., Shoemaker, R.C. & Palmer, R.G. Theoret. Appl. Genetics (1989) 77: 786. doi:10.1007/BF00268327

Summary

Fifty-eight soybean accessions from the genus Glycine, subgenus Soja, were surveyed with 17 restriction fragment length polymorphism (RFLP) genetic markers to assess the level of molecular diversity and to evaluate the usefulness of previously identified RFLP markers. In general, only low levels of molecular diversity were observed: 2 of the 17 markers exhibited three alleles per locus, whereas all others had only two alleles. Thirty-five percent of the markers had rare alleles present in only 1 or 2 of the 58 accessions. Molecular diversity was least among cultivated soybeans and greatest between accessions of different soybean species such as Glycine max (L.) Merr. and G. soja Sieb. and Zucc. Principal component analysis was useful in reducing the multidimensional genotype data set and identifying genetic relationships.

Key words

Restriction fragment length polymorphism Glycine max Glycine soja 

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • P. Keim
    • 1
  • R. C. Shoemaker
    • 1
    • 2
  • R. G. Palmer
    • 1
    • 2
  1. 1.Departments of Agronomy and GeneticsIowa State UniversityAmesUSA
  2. 2.USDA-Agricultural Research ServiceIowa State UniversityAmesUSA
  3. 3.Department of BiologyNorthern Arizona UniversityFlagstaffUSA

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