Applied Microbiology and Biotechnology

, Volume 28, Issue 4, pp 367–372

Comparison of Pseudomonas fragi and Gluconobacter oxydans for production of xylonic acid from hemicellulose hydrolyzates

  • Johanna Buchert
  • Jürgen Puls
  • Kaisa Poutanen
Biotechnology

DOI: 10.1007/BF00268197

Cite this article as:
Buchert, J., Puls, J. & Poutanen, K. Appl Microbiol Biotechnol (1988) 28: 367. doi:10.1007/BF00268197

Summary

Xylonic acid was produced efficiently from pure xylose by Pseudomonas fragi ATCC 4973 and Gluconobacter oxydans subsp. suboxydans ATCC 621. The yield from 10% xylose was in both cases over 95% of the theoretical. However, the sensitivities of the strains towards the major inhibitors found in hemicellulose hydrolyzates, ie. acetic acid, furfural and two lignin-derived compounds, varied. G. oxydans tolerated all these inhibitors better than P. fragi. In tests using steamed hemicellulose hydrolyzate, G. oxydans was able to utilize the substrate only at dilute xylose concentrations. After ether extraction or mixed bed resin pretreatment, the fermentability of the hydrolyzate was increased significantly.

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Johanna Buchert
    • 1
  • Jürgen Puls
    • 2
  • Kaisa Poutanen
    • 1
  1. 1.Biotechnical LaboratoryVTTEspooFinland
  2. 2.Institute of Wood Chemistry and Chemical Technology of WoodFederal Research Centre for Forestry and Forest ProductsHamburg 80Federal Republic of Germany