Histochemistry

, Volume 97, Issue 3, pp 269–275

Nuclear location of phosphoglycerate mutase BB isozyme in rat tissues

  • G. Egea
  • J. M. Ureña
  • X. Graña
  • J. Marsal
  • J. Carreras
  • F. Climent
Article

DOI: 10.1007/BF00267638

Cite this article as:
Egea, G., Ureña, J.M., Graña, X. et al. Histochemistry (1992) 97: 269. doi:10.1007/BF00267638
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Summary

We have previously reported (Ureña et al. Eur. J. Cell Biol. 1990) that in skeletal muscle, type MM phosphoglycerate mutase isozyme is present in the nucleus as well as in the cytosol. To determine whether type BB phosphoglycerate mutase isozyme is also present in nucleus, the subcellular location of this isozyme was studied in different rat tissues by cell fractionation and immunogold techniques. With the aid of high affinity-purified anti-phosphoglycerate mutase BB isozyme antibodies, the isozyme was located in the nucleus of neuronal, astroglial and liver cells but not in the nucleus of oligodendroglial and endothelial cells. Biochemical studies on purified nuclear fractions also demonstrated the presence of phosphoglycerate mutase activity in the nucleus. Both immunocytochemical and biochemical techniques showed that nuclear phosphoglycerate mutase-specific activity depended on the type of cell.

Abbreviations

PGAM

phosphoglycerate mutase

PGAM-M(M)

muscle specific subunit (isozyme) of PGAM

PGAM-B(B)

brain type subunit (isozyme) of PGAM

ssDNA

single stranded DNA

PBS

0.001 M phosphate buffer, pH 7.4, containing 0.15 M NaCl

kDa

kilodalton

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • G. Egea
    • 2
  • J. M. Ureña
    • 1
  • X. Graña
    • 1
  • J. Marsal
    • 2
  • J. Carreras
    • 1
  • F. Climent
    • 1
  1. 1.Unitat de Bioquimica. Facultat de MedicinaUniversitat de BarcelonaBarcelonaSpain
  2. 2.Departament de Biologia Cellular i Anatomia Patològica. Facultat de MedicinaUniversitat de BarcelonaBarcelonaSpain