Histochemistry

, Volume 93, Issue 1, pp 9–11

Improved bromodeoxyuridine/DNA analysis by anti-BudR monoclonal antibody versus right angle light scatter

  • M. Vitale
  • L. M. Neri
  • L. Manzoli
  • A. Galanzi
  • R. Rana
  • A. Antonucci
  • S. Papa
Article

DOI: 10.1007/BF00266840

Cite this article as:
Vitale, M., Neri, L.M., Manzoli, L. et al. Histochemistry (1989) 93: 9. doi:10.1007/BF00266840

Summary

Dynamic cell cycle analysis is based on the incorporation of labelled precursors into DNA. Although antibodies to BrdU are very useful for analysing in flow cells which synthesize DNA, this approach has two main limitations. First, the detection of low incorporating cells is often difficult; second, four parameter flow cytometry is not able to correlate cell cycle to any other cellular marker. We have developed a methodology that, employing an IgGH+L as a second antibody and side scatter instead of propidium iodide fluorescence, allows a better discrimination of BudR+ cells. This approach allows the collection of an extra-fluorescent signal, and the analysis of specific cellular markers within the cell cycle.

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • M. Vitale
    • 1
    • 4
  • L. M. Neri
    • 1
  • L. Manzoli
    • 1
  • A. Galanzi
    • 1
  • R. Rana
    • 2
  • A. Antonucci
    • 2
  • S. Papa
    • 3
    • 4
  1. 1.Istituto di Anatomia Umana NormaleUniversità di BolognaUrbinoItaly
  2. 2.Istituto di Morfologia UmanaUniversità di ChietiUrbinoItaly
  3. 3.Istituto di Scienze MorfologicheUniversità di UrbinoUrbinoItaly
  4. 4.Istituto di Citomorfologia Normale e PatologicaC.N.R.BolognaItaly