Molecular and General Genetics MGG

, Volume 154, Issue 1, pp 87–95

The isolation and preliminary characterisation of auxotrophic and analogue resistant mutants of the moss, Physcomitrella patens

  • N. W. Ashton
  • D. J. Cove
Article

DOI: 10.1007/BF00265581

Cite this article as:
Ashton, N.W. & Cove, D.J. Molec. Gen. Genet. (1977) 154: 87. doi:10.1007/BF00265581

Summary

Eighteen nutritional mutants have been isolated in the haploid, monoecious moss, Physcomitrella patens: five nicotinic acid auxotrophs, four p-aminobenzoic acid auxotrophs, four adenine auxotrophs, two amino acid requiring mutants and three nitrate non-utilising mutants. Seventeen of them were obtained using total isolation; one was isolated selectively. Strains resistant to the amino acid analogues, D-serine and p-fluorophenyl-alanine, and the purine analogue, 8-azaguanine, have been selected. Many of the auxotrophs are self-sterile. Crosses between auxotrophic strains have been effected and the progeny analysed. No linkage has been detected. Nicotinic acid auxotrophy has resulted from mutation in at least two genes. Self-sterility segregates as a pleiotropic effect of four mutations which produce nutritional dependence. A diploid strain has been obtained by aposporus regeneration from a hybrid sporophyte and the phenotypes of progeny resulting from the self-fertilisation of this strain have been analysed.

Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • N. W. Ashton
    • 1
  • D. J. Cove
    • 1
  1. 1.Department of GeneticsUniversity of CambridgeCambridgeEngland