Theoretical and Applied Genetics

, Volume 58, Issue 6, pp 257–263

Induction and analysis of gibberellin sensitive mutants in Arabidopsis thaliana (L.) heynh.

  • M. Koornneef
  • J. H. van der Veen
Article

DOI: 10.1007/BF00265176

Cite this article as:
Koornneef, M. & van der Veen, J.H. Theoret. Appl. Genetics (1980) 58: 257. doi:10.1007/BF00265176

Summary

In Arabidopsis thaliana 37 independent irradiation or EMS induced mutants were isolated which have an absolute or almost absolute gibberellin (GA) requirement for germination and successive elongation growth. These are called ‘non-germinating GA-dwarfs’, since without further addition of GA they develop into typical GA-dwarfs, being dark green, stunted and sterile. However, with repeated GA-treatment they develop into fertile plants with a completely wild type phenotype, or nearly so. In addition, 19 independently induced ‘germinating GA-dwarfs’ were obtained, i.e. mutants which do germinate without GA but develop into typical GA-dwarfs. With repeated GA-treatment these too grow to become completely wild type phenotypes, or nearly so. ‘Germinating dwarfs’ have been found by previous authors in a number of other plant species. The ‘non-germinating dwarfs’ form a new class of mutants. The system of non-germinating mutants offers a resolving power unique in higher plants, so that self-detecting rare events like induced revertants or intragenic recombinants can be efficiently screened for.

The 56 GA-sensitive mutants represent mutations at 5 loci, located on three of five Arabidopsis chromosomes. At three of the five loci both mutant classes were represented in similar frequency ratio's, whilst at the other two loci only germinating dwarfs were found.

Key words

Arabidopsis thalianaGibberellinGibberellin sensitive mutantsDwarf mutantsNon-germinating mutantsGene localization

Abbreviations

GA

gibberellin

EMS

ethylmethanesulfonate

NG

non-germinating

G

germinating

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • M. Koornneef
    • 1
  • J. H. van der Veen
    • 1
  1. 1.Department of GeneticsAgricultural UniversityWageningenThe Netherlands