Theoretical and Applied Genetics

, Volume 52, Issue 3, pp 135–139

Rapid estimation of potato tuber total protein content with coomassie brilliant blue G-250

  • J. C. Snyder
  • S. L. Desborough
Article

DOI: 10.1007/BF00264747

Cite this article as:
Snyder, J.C. & Desborough, S.L. Theoret. Appl. Genetics (1978) 52: 135. doi:10.1007/BF00264747

Summary

A new method for measuring protein with Coo-massie Brilliant Blue G-250 has been adapted for use as a screening method in a potato tuber protein improvement breeding program. The method is simple, fast and inexpensive, and has successfully estimated the total protein content of a broad range of tuber genotypes having dissimilar amino acid profiles and tuber maturities. Correlation between the Coomassie method and a modified micro-Kjeldahl method, the standard method used in the potato breeding program, was 0.93. Free amino acids and other compounds which interfere with other methods for measuring protein do not interfere with the Coomassie Brilliant Blue procedure.

Key words

Protein BreedingProtein ExtractionSolanum tuberosum L.Protein Selection MethodProtein Dye-Binding

Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • J. C. Snyder
    • 1
  • S. L. Desborough
    • 1
  1. 1.Department of Horticultural Science and Landscape ArchitectureUniversity of MinnesotaSt. PaulUSA