Molecular and General Genetics MGG

, Volume 229, Issue 1, pp 1–9

The KNS1 gene of Saccharomyces cerevisiae encodes a nonessential protein kinase homologue that is distantly related to members of the CDC28/cdc2 gene family


  • Ramesh Padmanabha
    • Department of BiologyYale University
  • Sonja Gehrung
    • Department of BiologyYale University
  • Michael Snyder
    • Department of BiologyYale University

DOI: 10.1007/BF00264206

Cite this article as:
Padmanabha, R., Gehrung, S. & Snyder, M. Molec. Gen. Genet. (1991) 229: 1. doi:10.1007/BF00264206


A novel protein kinase homologue (KNS1) has been identified in Saccharomyces cerevisiae. KNS1 contains an open reading frame of 720 codons. The carboxy-terminal portion of the predicted protein sequence is similar to that of many other protein kinases, exhibiting 36% identity to the cdc2 gene product of Schizosaccharomyces pombe and 34% identity to the CDC28 gene product of S. cerevisiae. Deletion mutations were constructed in the KNS1 gene. kns1 mutants grow at the same rate as wild-type cells using several different carbon sources. They mate at normal efficiencies, and they sporulate successfully. No defects were found in entry into or exit from stationary phase. Thus, the KNS1 gene is not essential for cell growth and a variety of other cellular processes in yeast.

Key words

Protein kinaseYeastCDC28Cell cycle

Copyright information

© Springer-Verlag 1991