Theoretical and Applied Genetics

, Volume 67, Issue 5, pp 463–467

Flow-cytometric characterization and sorting of plant chromosomes

  • A. M. M. de Laat
  • J. Blaas
Article

DOI: 10.1007/BF00263414

Cite this article as:
de Laat, A.M.M. & Blaas, J. Theoret. Appl. Genetics (1984) 67: 463. doi:10.1007/BF00263414

Summary

Flow cytometric measurements of DNA frequency distribution were used to follow the synchronization process in suspension cells from Haplopappus gracilis (2n=4). Metaphase chromosomes were isolated from these synchronized cells and both the acro- and metacentric chromosomes were sorted by flow cytometry based on the different DNA contents. Possible applications of this procedure in fundamental genetics as well as practical plant breeding are discussed.

Key words

Chromosome isolationFlow cytometryHaplopappus gracilis (Nutt.) GraySynchronization

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • A. M. M. de Laat
    • 1
  • J. Blaas
    • 1
  1. 1.Research Institute ITALAA WageningenThe Netherlands