Flow-cytometric characterization and sorting of plant chromosomes
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- de Laat, A.M.M. & Blaas, J. Theoret. Appl. Genetics (1984) 67: 463. doi:10.1007/BF00263414
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Flow cytometric measurements of DNA frequency distribution were used to follow the synchronization process in suspension cells from Haplopappus gracilis (2n=4). Metaphase chromosomes were isolated from these synchronized cells and both the acro- and metacentric chromosomes were sorted by flow cytometry based on the different DNA contents. Possible applications of this procedure in fundamental genetics as well as practical plant breeding are discussed.