Archives of Microbiology

, Volume 156, Issue 4, pp 239–247

Methanopyrus kandleri, gen. and sp. nov. represents a novel group of hyperthermophilic methanogens, growing at 110°C

Authors

  • Margit Kurr
    • Lehrstuhl für MikrobiologieUniversität Regensburg
  • Robert Huber
    • Lehrstuhl für MikrobiologieUniversität Regensburg
  • Helmut König
    • Abteilung Angewandte MikrobiologieUniversität Ulm
  • Holger W. Jannasch
    • Department of BiologyWoods Hole Oceanographic Institution
  • Hans Fricke
    • Max Planck-Institut für Verhaltensphysiologie
  • Antonio Trincone
    • Istituto per la Chimica di Molecole di Interesse Biologico
  • Jakob K. Kristjansson
    • Institute of BiologyUniversity of Iceland
  • Karl O. Stetter
    • Lehrstuhl für MikrobiologieUniversität Regensburg
Original Papers

DOI: 10.1007/BF00262992

Cite this article as:
Kurr, M., Huber, R., König, H. et al. Arch. Microbiol. (1991) 156: 239. doi:10.1007/BF00262992

Abstract

A novel group of hyperthermophilic rod-shaped motile methanogens was isolated from a hydrothermally heated deep sea sediment (Guaymas Basin, Gulf of California) and from a shallow marine hydrothermal system (Kolbeinsey ridge, Iceland). The grew between 84 and 110°C (opt: 98°C) and from 0.2% to 4% NaCl (opt. 2%) and pH 5.5 to 7 (opt: 6.5). The isolates were obligate chemolithoautotrophes using H2/CO2 as energy and carbon sources. In the presence of sulfur, H2S was formed and cells tended to lyse. The cell wall consisted of a new type of pseudomurein containing ornithin in addition to lysine and no N-acetylglucosamine. The pseudomurein layer was covered by a detergent-sensitive protein surface layer. The core lipid consisted exclusively of phytanyl diether. The GC content of the DNA was 60 mol%. By 16S rRNA comparisons the new organisms were not related to any of the three methanogenic lineages. Based on the physiological and molecular properties of the new isolates, we describe here a new genus, which we name Methanopyrus (the “methane fire”). The type species is Methanopyrus kandleri (type strain: AV19; DSM 6324).

Key words

Methanopyrus Methanogens Archaea Hyperthermophilic Marine Vents

Copyright information

© Springer-Verlag 1991