Original Articles

Urological Research

, Volume 17, Issue 2, pp 87-93

Structure and function of the androgen receptor

  • A. O. BrinkmannAffiliated withDepartment of Biochemistry II, Erasmus University
  • , P. KlaasenAffiliated withDepartment of Pathology, Erasmus University
  • , G. G. J. M. KuiperAffiliated withDepartment of Biochemistry II, Erasmus University
  • , J. A. G. M. van der KorputAffiliated withDepartment of Pathology, Erasmus University
  • , J. BoltAffiliated withDepartment of Biochemistry II, Erasmus University
  • , W. de BoerAffiliated withDepartment of Biochemistry II, Erasmus University
  • , A. SmitAffiliated withDepartment of Biochemistry II, Erasmus University
  • , P. W. FaberAffiliated withDepartment of Pathology, Erasmus University
  • , H. C. J. van RooijAffiliated withDepartment of Pathology, Erasmus University
    • , A. Geurts van KesselAffiliated withDepartment of Cell Biology and Genetics, Erasmus University
    • , M. M. VoorhorstAffiliated withDepartment of Biochemistry II, Erasmus University
    • , E. MulderAffiliated withDepartment of Biochemistry II, Erasmus University
    • , J. TrapmanAffiliated withDepartment of Pathology, Erasmus University

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Summary

The androgen receptor in several species (human, rat, calf) is a monomeric protein with a molecular mass of 100–110kDa. The steroid binding domain is confined to a region of 30 kDa, while the DNA-binding domain has the size of approx. 10 kDa. A 40 kDa fragment containing both the DNA and steroid binding domain displayed a higher DNA binding activity than did the intact 100 kDa molecule. cDNA encoding the major part of the human androgen receptor was isolated. The cDNA contains an open reading frame of 2,277 bp but still lacks part of the 5′-coding sequence. Homology with the progesterone and glucocorticoid receptor was about 80% in the DNA binding domain and 50% in the steroid binding domain. The present data provide evidence that the androgen receptor belongs to the superfamily of ligand responsive transcriptional regulators and consists of three distinct domains each with a specialized function.

Key words

Androgen Receptor cDNA Structure Human