Applied Microbiology

Applied Microbiology and Biotechnology

, Volume 23, Issue 3, pp 263-268

Elucidation of adsorption processes of cellulases during hydrolysis of crystalline cellulose

  • Mitsuo TanakaAffiliated withDepartment of Food Science and Technology, Faculty of Agriculture, Kyoto University
  • , Hiroko NakamuraAffiliated withDepartment of Food Science and Technology, Faculty of Agriculture, Kyoto University
  • , Masayuki TaniguchiAffiliated withDepartment of Food Science and Technology, Faculty of Agriculture, Kyoto University
  • , Takuo MoritaAffiliated withDepartment of Food Science and Technology, Faculty of Agriculture, Kyoto University
  • , Ryuichi MatsunoAffiliated withDepartment of Food Science and Technology, Faculty of Agriculture, Kyoto University
  • , Tadashi KamikuboAffiliated withDepartment of Food Science and Technology, Faculty of Agriculture, Kyoto University

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Summary

To elucidate the effect of adsorption of cellulases during hydrolysis of crystalline cellulose, the relationship between the rate of hydrolysis and the adsorption of crude cellulases onto crystalline cellulose was investigated under various experimental conditions. Several phases of adsorption have been proposed to explain the process of cellulose hydrolysis by these enzymes. The process of hydrolysis calculated on the basis of these phases fitted well with that obtained experimentally.