Theoretical and Applied Genetics

, Volume 72, Issue 1, pp 123–128

Bean arcelin

1. Inheritance of a novel seed protein of Phaseolus vulgaris L. and its effect on seed composition
  • J. Romero Andreas
  • B. S. Yandell
  • F. A. Bliss
Originals

DOI: 10.1007/BF00261467

Cite this article as:
Romero Andreas, J., Yandell, B.S. & Bliss, F.A. Theoret. Appl. Genetics (1986) 72: 123. doi:10.1007/BF00261467

Summary

SDS-PAGE of seed proteins from the seeds of a nondomesticated bean of Mexican origin (Phaseolus vulgaris L., PI 325690) revealed the presence of a novel 38 kd protein which appeared to be neither an altered phaseolin nor a lectin fraction. The protein was named arcelin, after Arcelia, the town in the state of Guerrero near which PI 325690 had been collected. The pure line, UW 325, was derived by self fertilization of the plant from a single arcelin-containing seed of PI 325690. Despite a low percentage seed phaseolin (14.6%), seed phenotype, seed germination, plant growth, pollen fertility, and percentage seed protein of UW 325 were normal. Analyses of F2 and F3 seeds from a single F1 plant of the cross ‘Sanilac’XPI 325690-3 revealed that arcelin expression was inherited as a single gene and that presence was dominant to absence of arcelin. The mean percentage phaseolin in the seeds of homozygous dominant Arc/Arc F3 families (14.0%) was significantly lower than that of the homozygous recessive arc/arc seeds (44.7%). The distribution of percentage phaseolin values for seeds within segregating families was bimodal and nonoverlapping. Without exception, seeds containing arcelin (Arc+phenotype) contained a lower percentage phaseolin than seeds lacking arcelin (Arc-phenotype). Although arcelin presence was associated with low percentage phaseolin, the Arc/Arc and Arc/arc genotypes were similar for seed weight and percentage total seed protein.

Key words

Phaseolus vulgaris L. Arcelin Phaseolin Seed proteins Genetic variation 

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • J. Romero Andreas
    • 1
  • B. S. Yandell
    • 1
  • F. A. Bliss
    • 1
  1. 1.Department of HorticultureUniversity of WisconsinMadisonUSA

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