Molecular and General Genetics MGG

, Volume 219, Issue 1, pp 119–124

Cloning and analysis of the nuclear genes for two mitochondrial ribosomal proteins in yeast

  • Yasuhiko Matsushita
  • Madoka Kitakawa
  • Katsumi Isono
Article

DOI: 10.1007/BF00261166

Cite this article as:
Matsushita, Y., Kitakawa, M. & Isono, K. Molec. Gen. Genet. (1989) 219: 119. doi:10.1007/BF00261166

Summary

Two mitochondrial ribosomal proteins of yeast (Saccharomyces cerevisiae) were purified and their N-terminal amino acid sequences determined. The sequence data were used for the synthesis of oligonucleotide probes to clone the corresponding genes. Thus, the genes for two proteins, termed YMR-31 and YMR-44, were cloned and their nucleotide sequences determined. From the nucleotide sequence data, the coding region of the gene for protein YMR-31 was found to be composed of 369 nucleotide pairs. Comparison of the amino acid sequence of protein YMR-31 and the one deduced from the nucleotide sequence of its gene suggests that it contains an octapeptide leader sequence. The calculated molecular weight of protein YMR-31 without the leader sequence is 12792 dalton. The gene for protein YMR-44 was found to contain a 147 bp intron which contains two sequences conserved among yeast introns. The length of the two exons flanking the intron totals 294 nucleotide pairs which can encode a protein with a calculated molecular weight of 11476 dalton. The gene for protein YMR-31 is located on chromosome VI, while the gene for protein YMR-44 is located on either chromosome XIII or XVI.

Key words

Yeast mitochondria Ribosomal proteins Nucleotide sequencing Intron 

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Yasuhiko Matsushita
    • 1
  • Madoka Kitakawa
    • 1
  • Katsumi Isono
    • 1
  1. 1.Department of Biology, Faculty of ScienceKobe UniversityRokkodai, KobeJapan

Personalised recommendations