New classes ofStreptomyces coelicolor A3(2) mutants blocked in undecylprodigiosin (Red) biosynthesis
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- Coco, E.A., Narva, K.E. & Feitelson, J.S. Molec. Gen. Genet. (1991) 227: 28. doi:10.1007/BF00260702
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Fifteen mutants ofStreptomyces coelicolor A3(2) blocked in both the bipyrrole branch (redA) and a second site specific to the undecylprodigiosin pathway were characterized. Some of the mutants were ordered biosynthetically based on cosynthesis experiments. Complementation of each of the mutants with wild-type DNA cloned in low- and high-copy number plasmid vectors allowed the mutants to be separated into 12 new classes which are physically clustered within approximately 37 kb on theS. coelicolor genome. Early-step biosynthetic genes are centrally located and are flanked by later-step and regulatory genes.