Molecular and General Genetics MGG

, Volume 227, Issue 1, pp 28–32

New classes ofStreptomyces coelicolor A3(2) mutants blocked in undecylprodigiosin (Red) biosynthesis

  • E. A. Coco
  • K. E. Narva
  • J. S. Feitelson
Article

DOI: 10.1007/BF00260702

Cite this article as:
Coco, E.A., Narva, K.E. & Feitelson, J.S. Molec. Gen. Genet. (1991) 227: 28. doi:10.1007/BF00260702

Summary

Fifteen mutants ofStreptomyces coelicolor A3(2) blocked in both the bipyrrole branch (redA) and a second site specific to the undecylprodigiosin pathway were characterized. Some of the mutants were ordered biosynthetically based on cosynthesis experiments. Complementation of each of the mutants with wild-type DNA cloned in low- and high-copy number plasmid vectors allowed the mutants to be separated into 12 new classes which are physically clustered within approximately 37 kb on theS. coelicolor genome. Early-step biosynthetic genes are centrally located and are flanked by later-step and regulatory genes.

Key words

Recombinant DNA Antibiotic production Genetic organization Directed mutant screen Prodigiosin 

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • E. A. Coco
    • 1
  • K. E. Narva
    • 1
  • J. S. Feitelson
    • 1
  1. 1.Medical Research DivisionAmerican Cyanamid CompanyPearl RiverUSA
  2. 2.Dept. of Molecular BiologyMycogen CorporationSan DiegoUSA