Molecular and General Genetics MGG

, Volume 227, Issue 1, pp 22–27

Inter- and intramolecular transposition of Tn903

  • Francoise Bernardi
  • Alberto Bernardi
Article

DOI: 10.1007/BF00260701

Cite this article as:
Bernardi, F. & Bernardi, A. Molec. Gen. Genet. (1991) 227: 22. doi:10.1007/BF00260701

Summary

We have performed a detailed analysis of intra-and intermolecular endproducts of transposition of the compound transposon Tn903 and we show that, in our system, the transposition activity is almost entirely driven by one of the flanking insertion sequences, IS903L. The relatively inactive state of IS903R can be conferred on IS903L by changing the orientation of the internal Tn region. IS903L mediates the formation of the majority of adjacent deletions, insertion/inversions nd cointegrates, all of which are representative of replicative transposition; only a very low level of conservative transposition can be observed. Our results are discussed in relation to those showing that Tn903 uses predominantly the conservative pathway.

Key words

Tn903Compound transposonsDeletionsInversions

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Francoise Bernardi
    • 1
  • Alberto Bernardi
    • 1
  1. 1.Laboratoire d'EnzymologieCNRSGif-sur-YvetteFrance