Molecular and General Genetics MGG

, Volume 226, Issue 3, pp 409–417

Genetic and molecular analysis of a cluster of rpf genes involved in positive regulation of synthesis of extracellular enzymes and polysaccharide in Xanthomonas campestris pathovar campestris

Authors

  • J. -L. Tang
    • The Sainsbury LaboratoryJohn Innes Centre for Plant Science Research
  • Y. -N. Liu
    • The Sainsbury LaboratoryJohn Innes Centre for Plant Science Research
  • C. E. Barber
    • The Sainsbury LaboratoryJohn Innes Centre for Plant Science Research
  • J. M. Dow
    • The Sainsbury LaboratoryJohn Innes Centre for Plant Science Research
  • J. C. Wootton
    • Department of GeneticsUniversity of Leeds
  • M. J. Daniels
    • The Sainsbury LaboratoryJohn Innes Centre for Plant Science Research
Article

DOI: 10.1007/BF00260653

Cite this article as:
Tang, J.-., Liu, Y.-., Barber, C.E. et al. Molec. Gen. Genet. (1991) 226: 409. doi:10.1007/BF00260653

Summary

The cosmid clone. pIJ3020 containing DNA from the plant pathogenic bacterium Xanthomonas campestris pathovar campestris has previously been shown to complement a non-pathogenic mutant defective in synthesis of extracellular enzymes. The DNA cloned in pIJ3020 was analysed by mutagenesis with Tn5 and Tn5lac and by nucleotide sequencing. The results indicate that this region of the genome contains a cluster of genes, mutation in any of which results in failure of the enzymes and extracellular polysaccharide to be synthesized. The designation rpf (regulation of pathogenicity factors) is proposed for these genes. The nucleotide sequence of one gene (rpfC) predicts a protein product with homology to conserved domains of both sensor and regulator proteins of prokaryotic two-component regulatory systems, which are usually involved in regulating gene expression in response to environmental stimuli.

Key words

Two-component regulatory systemPathogenicityXanthanDNA sequence

Copyright information

© Springer-Verlag 1991