Molecular and General Genetics MGG

, Volume 228, Issue 3, pp 486–490

Molecular cloning and nucleotide sequence of the Rhizobium phaseoli recA gene

  • Jan Michiels
  • Ann Vande Broek
  • Jos Vanderleyden
Short communication

DOI: 10.1007/BF00260644

Cite this article as:
Michiels, J., Vande Broek, A. & Vanderleyden, J. Molec. Gen. Genet. (1991) 228: 486. doi:10.1007/BF00260644

Summary

A recombinant λ phage carrying the recA gene of Rhizobium phaseoli was isolated from a R. phaseoli genomic library by complementation of the Fec phenotype of the recombinant phage in Escherichia coli. When expressed in E. coli, the cloned recA gene was shown to restore resistance to both UV irradiation and the DNA alkylating agent methyl methanesulphonate (MMS). The R. phaseoli recA gene also promoted homologous recombination in E. coli. The cloned recA gene was only weakly inducible in E. coli recA strains by DNA damaging agents. The DNA sequence of the R. phaseoli recA gene was determined and compared with published recA sequences. No LexA-binding site was detected in the R. phaseoli recA upstream region.

Key words

Rhizobium phaseoli RecA gene Recombination DNA sequence Fec 

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Jan Michiels
    • 1
  • Ann Vande Broek
    • 1
  • Jos Vanderleyden
    • 1
  1. 1.F.A. Janssens Laboratory of GeneticsUniversity of LeuvenHeverleeBelgium