Molecular and General Genetics MGG

, Volume 220, Issue 2, pp 301–306

Escherichia coli g1yA mRNA decay: The role of 3′ secondary structure and the effects of the pnp and rnb mutations

  • Michael D. Plamann
  • George V. Stauffer

DOI: 10.1007/BF00260498

Cite this article as:
Plamann, M.D. & Stauffer, G.V. Molec. Gen. Genet. (1990) 220: 301. doi:10.1007/BF00260498


The Escherichia coli glyA structural gene is followed by two REP sequences and a rho-independent transcription terminator. These sequences are essential for maintaining glyA mRNA stability and gene expression by blocking the 3′ to 5′ exonucleolytic activities of polynucleotide phosphorylase and ribonuclease II. The results support the model of cooperative endonucleolytic and 3′ to 5′ exonucleolytic activities in mRNA decay.

Key words

mRNA decay mRNA secondary structure glyA gene Polynucleotide phosphorylase Ribonuclease II 



glucose minimal


Luria broth


polynucleotide phosphorylase


repetitive extragenic palindromic

RNase II

ribonuclease II


serine hydroxymethyltransferase

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Michael D. Plamann
    • 1
  • George V. Stauffer
    • 1
  1. 1.Department of MicrobiologyUniversity of IowaIowa CityUSA
  2. 2.Department of Biological SciencesStanford UniversityStanfordUSA

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