Molecular and General Genetics MGG

, Volume 220, Issue 2, pp 301–306

Escherichia coli g1yA mRNA decay: The role of 3′ secondary structure and the effects of the pnp and rnb mutations

  • Michael D. Plamann
  • George V. Stauffer
Article

DOI: 10.1007/BF00260498

Cite this article as:
Plamann, M.D. & Stauffer, G.V. Molec. Gen. Genet. (1990) 220: 301. doi:10.1007/BF00260498

Summary

The Escherichia coli glyA structural gene is followed by two REP sequences and a rho-independent transcription terminator. These sequences are essential for maintaining glyA mRNA stability and gene expression by blocking the 3′ to 5′ exonucleolytic activities of polynucleotide phosphorylase and ribonuclease II. The results support the model of cooperative endonucleolytic and 3′ to 5′ exonucleolytic activities in mRNA decay.

Key words

mRNA decay mRNA secondary structure glyA gene Polynucleotide phosphorylase Ribonuclease II 

Abbreviations

GM

glucose minimal

LB

Luria broth

PNPase

polynucleotide phosphorylase

REP

repetitive extragenic palindromic

RNase II

ribonuclease II

SHMT

serine hydroxymethyltransferase

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Michael D. Plamann
    • 1
  • George V. Stauffer
    • 1
  1. 1.Department of MicrobiologyUniversity of IowaIowa CityUSA
  2. 2.Department of Biological SciencesStanford UniversityStanfordUSA

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