Applied Microbiology and Biotechnology

, Volume 23, Issue 5, pp 367–368

Isopenicillin N epimerase activity in a high cephalosporin-producing strain of Cephalosporium acremonium

  • Claus Lübbe
  • Saul Wolfe
  • Arnold L. Demain
Applied Microbiology

DOI: 10.1007/BF00257034

Cite this article as:
Lübbe, C., Wolfe, S. & Demain, A.L. Appl Microbiol Biotechnol (1986) 23: 367. doi:10.1007/BF00257034

Summary

Isopenicillin N epimerase activity in Cephalosporium acremonium CW-19 is so labile that it has never been detected in sonic extracts. Prior to this work, it had only been obtained by the laborious protoplast lysate procedure. The present work shows that the enzyme is present in sonic extracts of a high cephalosporin-producing strain (C-10) of C. acremonium throughout the fermentation.

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Claus Lübbe
    • 1
  • Saul Wolfe
    • 2
  • Arnold L. Demain
    • 1
  1. 1.Fermentation Microbiology Laboratory, Department of Applied Biological SciencesMassachusetts Institute of TechnologyCambridgeUSA
  2. 2.Department of ChemistryQueens UniversityKingstonCanada
  3. 3.Fermentation Process DevelopmentPharmaceutical Research DivisionUnionUSA