Applied Microbiology and Biotechnology

, Volume 22, Issue 6, pp 394–398

Production of acetone and butanol by Clostridium acetobutylicum in continuous culture with cell recycle

  • A. S. Afschar
  • H. Biebl
  • K. Schaller
  • K. Schügerl
Biotechnology

DOI: 10.1007/BF00252779

Cite this article as:
Afschar, A.S., Biebl, H., Schaller, K. et al. Appl Microbiol Biotechnol (1985) 22: 394. doi:10.1007/BF00252779
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Summary

To increase the solvent productivity of the acetone-butanol fermentation, a continuous culture of Clostridium acetobytylicum with cell recycling was used. At a dry cell mass concentration of 8 g l-1 and a dilution rate of D=0.64 h-1, a solvent productivity of 5.4 g l-1 h-1 was attained. To prevent degeneration of the culture, which occurs with high concentrations of solvents (acetone, butanol and ethanol), different reactor cascades were used. A two-stage cascade with cell recycling and turbidostatic cell concentration control turned out to be the best solution, the first stage of which was kept at relatively low cell and product concentrations. A solvent productivity of 3 and 2.3 g l-1 h-1, respectively, was achieved at solvent concentrations of 12 and 15 g l-1.

Symbols

D

Dilution rate (h-1)

rp

solvent productivity (g l-1 h-1)

s

residual glucose concentration (g l-1)

VR

reactor volume (l)

VO

overall volume (l)

x

(dry) cell mass concentration (g l-1)

YP/S

solvent yield (g g-1)

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • A. S. Afschar
    • 1
  • H. Biebl
    • 1
  • K. Schaller
    • 1
  • K. Schügerl
    • 2
  1. 1.Gesellschaft für Biotechnologische Forschung mbHBraunschweigFederal Republic of Germany
  2. 2.Institut für Technische ChemieUniversität HannoverHannoverFederal Republic of Germany