Applied Microbiology and Biotechnology

, Volume 22, Issue 6, pp 394-398

First online:

Production of acetone and butanol by Clostridium acetobutylicum in continuous culture with cell recycle

  • A. S. AfscharAffiliated withGesellschaft für Biotechnologische Forschung mbH
  • , H. BieblAffiliated withGesellschaft für Biotechnologische Forschung mbH
  • , K. SchallerAffiliated withGesellschaft für Biotechnologische Forschung mbH
  • , K. SchügerlAffiliated withInstitut für Technische Chemie, Universität Hannover

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To increase the solvent productivity of the acetone-butanol fermentation, a continuous culture of Clostridium acetobytylicum with cell recycling was used. At a dry cell mass concentration of 8 g l-1 and a dilution rate of D=0.64 h-1, a solvent productivity of 5.4 g l-1 h-1 was attained. To prevent degeneration of the culture, which occurs with high concentrations of solvents (acetone, butanol and ethanol), different reactor cascades were used. A two-stage cascade with cell recycling and turbidostatic cell concentration control turned out to be the best solution, the first stage of which was kept at relatively low cell and product concentrations. A solvent productivity of 3 and 2.3 g l-1 h-1, respectively, was achieved at solvent concentrations of 12 and 15 g l-1.