Applied Microbiology and Biotechnology

, Volume 20, Issue 2, pp 124–128

Characterization of Schwanniomyces castellii mutants with increased productivity of amylases

  • A. M. Sills
  • P. S. J. Zygora
  • G. G. Stewart
Biotechnology

DOI: 10.1007/BF00252589

Cite this article as:
Sills, A.M., Zygora, P.S.J. & Stewart, G.G. Appl Microbiol Biotechnol (1984) 20: 124. doi:10.1007/BF00252589

Summary

The production of α-amylase activity in the yeast Schwanniomyces castellii strain 1402 is repressed in the presence of the non-metabolizable glucose analogue, 2-deoxy-glucose. Selection for resistance to 2-deoxy-glucose after treatment with ethyl methane sulphonate (EMS) or UV light has yielded mutants displaing increased α-amylase activities. One such mutant, S. castellii strain 1436, was found to exhibit constitutive α-amylase activity in glucose-containing medium. This constitutive enzyme activity was also observed under pilot scale fermentation conditions when the pH was maintained constant at 5.5±0.1. The disaccharide maltose served as a stronger inducer of α-amylase activity than the natural substrate starch in both the wild type (1402) and mutant (1436) strains.

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • A. M. Sills
    • 1
  • P. S. J. Zygora
    • 1
  • G. G. Stewart
    • 1
  1. 1.Production Research DepartmentLabatt Brewing Company Ltd.LondonCanada