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Elevated uptake of 67Ga and increased heparan sulfate content in liver-damaged rats

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Abstract

Values of percent 67Ga binding with standard acid mucopolysaccharides (AMPS) were determined. The binding percent of 67Ga with heparan sulfate (HS) was especially high. The relation between 67Ga and HS content was investigated in liver-damaged rats. Elevated uptake of 67Ga occurred in two stages, corresponding to the early and the later stages during feeding with 2-acetylaminofluorene (AAF). Uptake of 67Ga in the liver was first observed at 5–6 weeks after the start of AAF feeding. There was a decrease at about week 7 almost to the control level, followed by a sustained increase. This changing pattern of 67Ga uptake was in good accord with that of liver HS content. A similar relation between 67Ga uptake and HS content was also observed in rat liver treated with a single dose of carbon tetrachloride (CCl4). The elevated 67Ga uptake and increased HS content induced by CCl4 were both significantly inhibited by aminoacetonitrile (AAN) or papain pretreatment.

Moreover, we screened cations having a high affinity with HS from the exchangeability of 45Ca with those in 45Ca-HS complex. Fe3+ and other trivalent cations such as Al3+, La3+, Ce3+, Er3+, Sm3+, Yb3+, In3+, Ru3+, and Eu3+, and Le2+ represented strong affinity to HS. These cations also effectively decreased the 67Ga tissue distribution in normal rats. These results suggest that HS may be involved in the mechanism of 67Ga uptake in tumor and inflammatory tissues.

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Authors and Affiliations

  1. Department of Radiopharmacy, School of Pharmaceutical Sciences, Teikyo University, Suarashi 1091-1, Sagamiko-machi, Tukui-gun, 199-01, Kanagawa, Japan

    Shuji Kojima, Yukie Hama & Toru Sasaki

  2. Department of Radiopharmacy, School of Pharmaceutical Sciences, Science University of Tokyo, Funagawara-cho 12, Ichigaya, Shinjuku-ku, 162, Tokyo, Japan

    Akiko Kubodera

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  1. Shuji Kojima
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  2. Yukie Hama
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  3. Toru Sasaki
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  4. Akiko Kubodera
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Kojima, S., Hama, Y., Sasaki, T. et al. Elevated uptake of 67Ga and increased heparan sulfate content in liver-damaged rats. Eur J Nucl Med 8, 52–59 (1983). https://doi.org/10.1007/BF00252556

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