, Volume 31, Issue 1, pp 61-64

Optimization of cultivation medium composition for isoamylase production

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The medium composition for production of isoamylase by Pseudomonas amyloderamosa JD210 was optimized using response surface methodology. The factors chosen for optimization were maltose, soybean protein hydrolysate (SPH), isoleucine, proline, KH2PO4 and MgSO4. Fractional factorial designs (FFD) and the path of steepest ascent were effective in searching for the major factors and optimum medium composition. By a 26–1 FFD, supplementary isoleucine was shown to have a negative effect on enzyme production. The effects of the other five factors were further investigated by a central composite design and the optimum composition was found to be 1.10% maltose, 0.13% SPH, 0.15% proline, 0.38% KH2PO4, and 0.05% MgSO4. When the strain was cultivated in the optimum medium, enzyme production increased 60% compared with ordinary medium. Proline was verified as being a significant factor in promoting enzyme production.