Hudson River sediments as a source of actinomycetes exhibiting antifungal activity
- Cite this article as:
- Pisano, M.A., Sommer, M.J. & Brett, B.P. Appl Microbiol Biotechnol (1987) 27: 214. doi:10.1007/BF00251947
Actinomycetes were isolated from sediments obtained from the Hudson River. Pretreatments utilized to improve the recovery of these microorganisms included heat and exposure to phenol or benzalkonium chloride. In addition, plating of sediment samples on selective agar substrates was also employed. These pretreatments eliminated or severely limited the growth of contaminating microorganisms thereby facilitating the isolation of actinomycetes. Of 165 isolates obtained, 22 exhibited significant antimycotic activity following growth in submerged culture. Among the test fungi examined, Candida krusei and Trichoderma viride proved to be the most susceptible to the active substances present in the fermentation broths. All but three of the latter contained polyenes. With one exception, the bioactive actinomycetes were identified as streptomycetes.