Applied Microbiology and Biotechnology

, Volume 28, Issue 3, pp 268–271

Use of protoplast fusion to introduce methionine overproduction into Saccharomyces cerevisiae

  • Patrizia Brigidi
  • Diego Matteuzzi
  • Fabio Fava
Applied Genetics and Regulation

DOI: 10.1007/BF00250453

Cite this article as:
Brigidi, P., Matteuzzi, D. & Fava, F. Appl Microbiol Biotechnol (1988) 28: 268. doi:10.1007/BF00250453

Summary

dl-Ethionine-resistant mutants of Saccharomyces uvarum ATCC 26602 were found to overproduce exogenous l-methionine. dl-Ethionine-resistant mutant ER 108, carrying a mutation to chloramphenicol resistance was converted to petite form, and protoplasts obtained from it were fused with protoplasts from antibiotic-sensitive S. cerevisiae X2928 carrying six auxotrophies. The resulting fusants maintained four auxotrophies and were capable of overproducing l-methionine. These fusants were stable after ten passages on complete medium.

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Patrizia Brigidi
    • 1
  • Diego Matteuzzi
    • 1
  • Fabio Fava
    • 1
  1. 1.Cattedra di Chimica delle Fermentazioni e Microbiologia Industriale, Facoltà di FarmaciaUniversità di BolognaBolognaItaly