Use of protoplast fusion to introduce methionine overproduction into Saccharomyces cerevisiae
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- Brigidi, P., Matteuzzi, D. & Fava, F. Appl Microbiol Biotechnol (1988) 28: 268. doi:10.1007/BF00250453
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dl-Ethionine-resistant mutants of Saccharomyces uvarum ATCC 26602 were found to overproduce exogenous l-methionine. dl-Ethionine-resistant mutant ER 108, carrying a mutation to chloramphenicol resistance was converted to petite form, and protoplasts obtained from it were fused with protoplasts from antibiotic-sensitive S. cerevisiae X2928 carrying six auxotrophies. The resulting fusants maintained four auxotrophies and were capable of overproducing l-methionine. These fusants were stable after ten passages on complete medium.