, Volume 154, Issue 1, pp 82-84

Detection and determination of factor C — a regulatory protein —in Streptomyces strains by antiserum and monoclonal antibody

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Abstract

Rabbit antisera and monoclonal antibodies were raised against factor C, a regulatory protein of Streptomyces griseus. ELISA and immunoblotting techniques suitable to determine and characterize factor C antigen in bacterial specimens were developed. Factor C antigen was detected in all the 23 Streptomyces strains and variants examined thus far and in one Bacillus subtilis too. Depending on the strain analysed it has a molecular mass of 34 000 or 70 000 in mycelial homogenates. Most of factor C was found excreted into the cultivation medium. The quantity of factor C antigen in different Streptomyces strains showed great variation. Amy + strains were usually good producers of factor C while Amy were not. This was consistent with our assumption that factor C was an inducer of reproductive phase in Streptomyces.

Parts of experimental material reported here have been presented at the Fifth International Symposium on the Genetics of Industrial Microorganisms, Split, 1986; at the 208th Meeting of the Genetical Society, Norwich, 1988 and at the Seventh International Symposium on Biology of Actinomycetes, Tokyo, 1988