Experimental Brain Research

, Volume 69, Issue 1, pp 43–52

Spontaneous neuronal firing patterns in fetal rat cortical networks during development in vitro: a quantitative analysis

  • A. M. M. C. Habets
  • A. M. J. Van Dongen
  • F. Van Huizen
  • M. A. Corner
Article

DOI: 10.1007/BF00247027

Cite this article as:
Habets, A.M.M.C., Van Dongen, A.M.J., Van Huizen, F. et al. Exp Brain Res (1987) 69: 43. doi:10.1007/BF00247027

Summary

The development of spontaneous bioelectric activity (SBA) was studied in dissociated occipital cortex cultures prepared from 19 day old rat fetuses. All cultures, recorded one per diem from 5 to 33 days in vitro (div), showed SBA. Computer analysis of 76 extracellularly recorded single unit spike trains was carried out after selection on the basis of stationarity criteria. Statistically significant developmental trends were found in (i) interspike interval dependencies and (ii) fluctuations in mean firing rate, on the order of a minute or longer. The highly dependent firing patterns, including stereotyped bursting, were present mostly in the 9–12 div group, whereas minute-to-minute fluctuations in the intensity of firing were considerably more pronounced in the oldest group (22–33 div) than in the younger cultures. In addition, firing categories defined on the basis of factor-analysis revealed that such fluctuations were almost exclusively to be found in neurons which fired in a pronounced ‘burst’, rather than a relatively continuous fashion. Only a few mature appearing synaptic structures were observed electron microscopically prior to 12 div, but increased steadily in number thereafter. No cultures prior to 14 div, but all cultures older than this, stained positively for the presence of glutamic acid decarboxylase. An extensive immunoreactive, putative GABAergic, network was present by three weeks in vitro.

Key words

Spike train analysisSpontaneous activityPrimary cultureNeuronal developmentOccipital cortexRat

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • A. M. M. C. Habets
    • 1
  • A. M. J. Van Dongen
    • 1
  • F. Van Huizen
    • 1
  • M. A. Corner
    • 1
  1. 1.Netherlands Institute for Brain Research, I.W.O.AZ AmsterdamThe Netherlands
  2. 2.International Research and Science CenterNC Kerkrade-WestThe Netherlands
  3. 3.Department of Physiology and Molecular BiophysicsBaylor College of Medicine, Texas Medical CenterHoustonUSA
  4. 4.Department of PsychologyDalhousie UniversityHalifaxCanada