Archives of Microbiology

, Volume 155, Issue 6, pp 607–612

Studies on the utilization of lactose by Corynebacterium glutamicum, bearing the lactose operon of Escherichia coli

  • W. Brabetz
  • W. Liebl
  • K.-H. Schleifer
Original Papers

DOI: 10.1007/BF00245357

Cite this article as:
Brabetz, W., Liebl, W. & Schleifer, KH. Arch. Microbiol. (1991) 155: 607. doi:10.1007/BF00245357

Abstract

The entire Escherichia coli lactose operon was inserted into an E. coli/Corynebacterium glutamicum shuttle vector and introduced into the gram-positive host organism C. glutamicum R163. Recombinant C. glutamicum strains carrying the lac genes downstream of an efficient promoter displayed rapid growth with lactose as the sole source of carbon. Two prerequisites were necessary to obtain good growth of C. glutamicum R 163 on lactose: presence of the lacY gene in addition to lacZ and an appropriatc promoter for efficient transcription in C. glutamicum. The galactose moiety of lactose was not utilized but accumulated in the culture broth. C. glutamicum strains carrying only the lacZ (β-galactosidase) gene but not lacY (lactose permease) were not able to grow in lactose minimal medium.

Key words

Corynebacterium glutamicumLactose utilizationβ-galactosidaseLactose permeaseGalactose accumulation

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • W. Brabetz
    • 1
  • W. Liebl
    • 1
  • K.-H. Schleifer
    • 1
  1. 1.Lehrstuhl für MikrobiologieTechnische Universität MünchenMünchen 2Federal Republic of Germany