, Volume 191, Issue 1, pp 137-142

α-1,4-Glucan lyase, a new class of starch/glycogen-degrading enzyme

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Abstract

Antibodies have been raised against an α-1,4-glucan lyase purified from the red alga Gracilariopsis lemaneiformis (Bory) Dawson, Acleto et Foldvik. Localization of α-1,4-glucan lyase in ultra-thin sections of the red alga was performed using immunogold/transmission electron microscopy. The enzyme was found exclusively in the stroma of the chloroplasts of the algal cells, not in the cell wall, cytosol or around the cytosolic starch granules. Partial amino-acid sequences of the algal lyase, with a total length of 100 amino-acid residues, were obtained. No sequence homology was found with proteins and peptides of known sequences.

This work was supported by grants from the Swedish Council for Planning and Coordination of Research (FRN), Carl Tryggers Foundation, and Hierta-Retzius Fund. We thank Ms Katrin Österlund and Anette Axén for their expert technical assistance with this work.