Planta

, Volume 191, Issue 1, pp 137–142

α-1,4-Glucan lyase, a new class of starch/glycogen-degrading enzyme

II. Subcellular localization and partial amino-acid sequence
  • Shukun Yu
  • Marianne Pedersén
Article

DOI: 10.1007/BF00240905

Cite this article as:
Yu, S. & Pedersén, M. Planta (1993) 191: 137. doi:10.1007/BF00240905

Abstract

Antibodies have been raised against an α-1,4-glucan lyase purified from the red alga Gracilariopsis lemaneiformis (Bory) Dawson, Acleto et Foldvik. Localization of α-1,4-glucan lyase in ultra-thin sections of the red alga was performed using immunogold/transmission electron microscopy. The enzyme was found exclusively in the stroma of the chloroplasts of the algal cells, not in the cell wall, cytosol or around the cytosolic starch granules. Partial amino-acid sequences of the algal lyase, with a total length of 100 amino-acid residues, were obtained. No sequence homology was found with proteins and peptides of known sequences.

Key words

α-1,4-Glucan lyase (immunogold localization) Rhodophyta Starch/glycogen degradation 

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Shukun Yu
    • 1
  • Marianne Pedersén
    • 1
  1. 1.Department of Physiological BotanyUppsala UniversityUppsalaSweden

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