Planta

, Volume 197, Issue 1, pp 57–62

The contributions of adenosine 5′-diphosphoglucose pyrophosphorylase and starch-branching enzyme to the control of starch synthesis in developing pea embryos

  • Kay Denyer
  • Jonathan Foster
  • Alison M. Smith
Article

DOI: 10.1007/BF00239939

Cite this article as:
Denyer, K., Foster, J. & Smith, A.M. Planta (1995) 197: 57. doi:10.1007/BF00239939

Abstract

The contributions of ADP-glucose pyrophosphorylase (EC 2.7.7.27) and starch-branching enzyme (EC 2.4.1.18) to the control of the rate of starch synthesis in developing pea (Pisum sativum L.) embryos was investigated. Estimates were made of the deviation indices for changes in the activities of these enzymes at a point approximately halfway through embryo development, when starch was accumulating rapidly. The values for the deviation indices, and thus the flux control coefficients, were 0.1 for ADP-glucose pyrophosphorylase and 0.05 for starch-branching enzyme. Both values are low, suggesting that neither enzyme is of overriding importance in the control of starch synthesis in pea embryos.

Key words

ADP-glucose pyrophosphorylaseFlux control analysis (starch synthesis)Pisum (developing embryo)Plastid (pea embryo)Starch-branching enzymeStarch synthesis (plant embryo)

Abbreviations

ADPGpp

ADP-glucose pyrophosphorylase (adenosine 5′-diphosphoglucose pyrophosphorylase)

SBE

starchbranching enzyme (1,4-α-d-glucan-6-glycosyl transferase)

SSS

soluble starch synthase (ADP glucose: 1,4-α-d-glucan-4-glucosyl transferase)

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Kay Denyer
    • 1
  • Jonathan Foster
    • 1
  • Alison M. Smith
    • 1
  1. 1.John Innes CentreNorwichUK